TABLE 2.
List of identified heat shock proteins of B. cereus ATCC 14579
| HSP no. | Designation | N-terminal sequencea | Molecular massb (kDa) | Description | % Identity (closest hit) |
|---|---|---|---|---|---|
| 1c | DnaK | SKIIGIID | 72 | Heat shock chaperone | 83 (B. subtilis) |
| |||||||| | |||||
| SKIIGIID | |||||
| 3 | GroEL | MAKDIKFSEEA | 60 | 60-kDa chaperonin | 89 (B. subtilis) |
| ||||||||||| | |||||
| MAKDIKFSEEA | |||||
| 4 | YbbT | XXKYFGTD | 48 | Phosphoglucosamine mutase | 78 (B. subtilis) |
| |||||| | |||||
| MGKYFGTD | |||||
| 5 | AldA | MRXGIPXE | 40 | Alanine dehydrogenase | 73 (B. sphaericus) |
| |||||| | |||||
| MRIGIPTE | |||||
| 7 | MreB | MFGFXGF | 36 | Rod shape-determining protein | 84 (B. subtilis) |
| |||||| | 36 | ||||
| MFGFGGF | |||||
| 10 | FolD | XVAVIIKG | 31 | Methylene tetrahydrofolate dehydrogenase | 68 (B. subtilis) |
| ||||||| | |||||
| MVAVIIKG | |||||
| 11 | Dra | MNAIKLI | 24 | Deoxyribose phosphate aldolase | 76 (B. subtilis) |
| ||||||| | |||||
| MNIAKLI | |||||
| 12 | SodA | XAKHELPN | 23 | Superoxide dismutase | 79 (B. stearothermophilus) |
| ||||||| | |||||
| MAKHELPN | |||||
| 14 | ClpP | MNLIPTVIEQ | 21 | ATP-dependent protease | 90 (B. subtilis) |
| |||||||||| | |||||
| MNLIPTVIEQ | |||||
| 17 | HSP16.4 | MRNLFPE | 16 | Heat shock chaperone | 35 (S. thermophilus) |
| ||||||| | |||||
| MRNLFPE | |||||
| 18 | PpiB | MKTLGYI | 16 | Peptidyl-prolyl cis-trans isomerase | 74 (B. halodurans) |
| ||||||| | |||||
| MKTLGYI | |||||
| 19 | YflT | METXYRK | 17 | General stress protein 17M | 47 (B. subtilis) |
| |||||| | |||||
| METKYRK | |||||
| 23 | RsbV | MNLAIN | 12 | Anti-sigma factor B antagonist | 95 (B. anthracis) |
| |||||| | |||||
| MNLAIN | |||||
| 24 | SpoVG | MEVTDVR | 10 | Stage V sporulation protein G | 85 (B. subtilis) |
| ||||||| | |||||
| MEVTDVR | |||||
| 25 | TrxA | XAIVNAND | 11 | Thioredoxin | 72 (B. halodurans) |
| ||||||| | |||||
| MAIVNAND | |||||
| 27 | YloH | MLXP | 7 | RNA polymerase omega subunit | 67 (B. subtilis) |
| ||| | |||||
| MLNP | |||||
| 30 | CspE | MQGKVK | 7 | Cold shock protein E | 92 (B. cereus) |
| |||||| | |||||
| MQGKVK | |||||
| 31 | CspB | MTLTGKV | 71 | Cold shock protein B | 97 (B. cereus) |
| ||||||| | |||||
| MTLTGKV |
a
The upper sequence is the derived N-terminal sequence, and the lower sequence represents the N-terminal sequence of the respective protein in the B. cereus ATCC 14579 genome. The letter X indicates an unknown residue.
b
Calculated molecular mass based on the encoded gene sequence.
c
The N-terminal sequences of proteins 2, 6, 8, 9, 13, 15, 16, 20, 21, 22, 26, 28, and 29 have not been determined.