TABLE 1.
Plasmids used in this study
| Plasmid | Description/use | Source |
|---|---|---|
| pUBH | Genomic clone of PII | 1 |
| pAotubF2 | Gene fragment of tubA (from A. oligospora) | This study |
| pBHE | Gene disruption vector for PII | This study |
| pCB1003 | Transformation vector containing hph | 5 |
| pAN7-1 | Transformation vector containing hph | 19 |
| pCB1003-PII | Transformation vector containing hph and PII | This study |
| pAFR | PCR clone of PII | This study |
| pAN52-7Not | glaA promoter (from A. niger) | 30 |
| pASP | gluA promoter linked to PII | This study |
| pAMDSPYRG#1 | amdS/pyrG selection marker | (P. J. Punt, unpublished data) |