TABLE 2.
Diagnostic value of different assays for prenatal diagnosis of congenital infection in 88 fetuses of 86 mothers with primary HCMV infection in pregnancya
| Clinical specimen | HCMV assay | Test result | No. of fetuses
|
Sensitivity (%) | Specificity (%) | PPV (%) | NPV (%) | |
|---|---|---|---|---|---|---|---|---|
| Infected | Uninfected | |||||||
| Fetal blood | Antigenemia | Neg | 13 | 35 | 63.9 | 100 | 100 | 72.9 |
| Pos | 23 | 0 | ||||||
| Viremia | Neg | 17 | 31 | 41.4 | 100 | 100 | 64.6 | |
| Pos | 12 | 0 | ||||||
| DNAemia | Neg | 5 | 27 | 84.8 | 100 | 100 | 84.4 | |
| Pos | 28 | 0 | ||||||
| Immediate-early mRNA | Neg | 2 | 11 | 84.6 | 100 | 100 | 84.6 | |
| Pos | 11 | 0 | ||||||
| pp67 mRNA | Neg | 4 | 11 | 63.6 | 100 | 100 | 73.3 | |
| Pos | 7 | 0 | ||||||
| IgM antibody | Neg | 15 | 36 | 57.1 | 100 | 100 | 70.6 | |
| Pos | 20 | 0 | ||||||
| Amniotic fluid | Virus isolation | Neg | 8 | 44 | 81.8 | 100 | 100 | 84.6 |
| Pos | 36 | 0 | ||||||
| DNA | Neg | 3 | 38 | 92.7 | 100 | 100 | 92.7 | |
| Pos | 38 | 0 | ||||||
| Immediate-early mRNA | Neg | 2 | 32 | 92.6 | 97 | 96 | 94.1 | |
| Pos | 25 | 1 | ||||||
| pp67 mRNA | Neg | 2 | 33 | 92.0 | 100 | 100 | 94.3 | |
| Pos | 23 | 0 | ||||||
a
neg, negative; pos, positive; PPV, positive predictive value; NPV, negative predictive value. Source: M. G. Revello and G. Gerna, unpublished data.