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Strains and plasmids used in the study
| Strain or plasmid | Characteristics |
|---|---|
| S. aureus | |
| COL | Laboratory strain, Mcr |
| COL-VR1 | Vancomycin-resistant mutant from COL |
| E. coli | |
| HK4457 | E. coli BL21/pHK4457 |
| HK4386 | E. coli BL21/pHK4386 |
| HK4476 | E. coli M15(pREP4)/pHK4476 |
| Plasmids | |
| pHK4457 | pbpA gene cloned into pGEX-3T vector |
| pHK4386 | pbpD gene cloned into FLAG-MAC vector |
| pHK4476 | mecA gene cloned into pQE30 vector |
