| Component | Amount per well |
|---|---|
| PASTE Plasmid (e.g. pDY0216) or pDY0191 (negative control) | 100ng |
| atgRNA F | 20ng |
| atgRNA R | 20ng |
| Payload plasmid (e.g. pDY0181) | 180ng |
| p3000 | 0.66μL |
| Opti-MEM | To 5μL |
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