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. Author manuscript; available in PMC: 2025 Dec 13.
Published in final edited form as: Nat Protoc. 2024 Dec 15;20(6):1546–1583. doi: 10.1038/s41596-024-01090-z
Component Amount per reaction (μL) Final concentration
NebNext PCR Master Mix (2x) 2.5 1x
Primer pool F (10μM each; see Table 1) 0.25 0.5μM
Primer R 1 (100μM; see Table 1) 0.025 0.5μM
pDY0181_GFP_insert_NGS_R (PD6893) (100μM; see Table 1) 0.025 0.5μM
UltraPure water To 4.5μL