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. Author manuscript; available in PMC: 2025 Dec 13.
Published in final edited form as: Nat Protoc. 2024 Dec 15;20(6):1546–1583. doi: 10.1038/s41596-024-01090-z
Component Amount per reaction (μL) Final concentration
ddPCR Supermix for Probes (2x) 12 1x
Primer F (100μM; see Table 1) 0.1 0.4μM
Primer R (100μM; see Table 1) 0.1 0.4μM
FAM probe (100μM; see Table 1) 0.05 0.2μM
ddPCR HEX reference probe (20x) 1.2 1x
FastDigest restriction enzyme 1 0.1
FastDigest restriction enzyme 2 (optional) 0.1
UltraPure water To 24μL