| Component | Amount per reaction (μL) | Final concentration |
|---|---|---|
| ARCA/NTP 2x Mix (NEB) | 10 | 1x |
| Template DNA (from Step 4 of this Box) 250ng/μL | 4 | 50ng/μL |
| Pseudouridine-5′-triphosphate 100mM (Trilink) | 0.25 | 1.25mM |
| T7 RNA polymerase mix (NEB) | 2 | 1x |
| Murine RNase inhibitor (NEB) | 0.5 | 1000U/μL |
| UltraPure water | To 20μL |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.