| Component | Amount per reaction (μL) | Final concentration |
|---|---|---|
| IVT reaction (from Step 9 of this Box) | 22 | |
| 10x Poly(A) Polymerase reaction buffer | 5 | 1x |
| Poly(A) Polymerase | 5 | |
| UltraPure water | To 50μL |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.