| Steps 1–19, | Cloning guide RNAs: 4d |
| Steps 20–29, | Cloning custom payload into acceptor plasmid: optional): 4d |
| Steps 30–46, | Transfection of atgRNA combinations: 3d |
| Steps 47–61, | Reading out attB insertion by 2-primer NGS: 2d |
| Steps 63–65, | Transfection with payload plasmid: 3d |
| Steps 66–67, | 3-primer NGS assay: 2d |
| Steps 68–73, | Analysis of NGS: 1d |
| Steps 74–91, | Assessing payload integration by ddPCR: 1–2d |
| Box 3, Production of in vitro transcribed PASTE mRNA: optional): 1d | |
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