| Component | Amount per reaction (μL) | Final concentration |
|---|---|---|
| NebNext PCR Master Mix (2x) | 12.5 | 1x |
| Nicking guide PCR F (50μM) | 0.1 | 0.2μM |
| Nicking guide PCR R (universal) (50μM) | 0.1 | 0.2μM |
| Template (10ng/μL) | 1 | |
| UltraPure water | 11.3 | |
| Total volume | 25 |
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