Table 2.

Second round NGS barcode primers

NGS round 2 PCR forward primers		Step
NGS_96_F_A1	AATGATACGGCGACCACCGAGATCTACACAAGTAGAGACACTCTTTCCCTACACGACGCTCTTCC	53, 67
NGS_96_F_A2	AATGATACGGCGACCACCGAGATCTACACCATGCTTAACACTCTTTCCCTACACGACGCTCTTCC	53, 67
NGS_96_F_A3	AATGATACGGCGACCACCGAGATCTACACGCACATCTACACTCTTTCCCTACACGACGCTCTTCC	53, 67
NGS_96_F_A4	AATGATACGGCGACCACCGAGATCTACACTGCTCGACACACTCTTTCCCTACACGACGCTCTTCC	53, 67
NGS_96_F_A5	AATGATACGGCGACCACCGAGATCTACACAGCAATTCACACTCTTTCCCTACACGACGCTCTTCC	53, 67
NGS_96_F_A6	AATGATACGGCGACCACCGAGATCTACACAGTTGCTTACACTCTTTCCCTACACGACGCTCTTCC	53, 67
NGS_96_F_A7	AATGATACGGCGACCACCGAGATCTACACCCAGTTAGACACTCTTTCCCTACACGACGCTCTTCC	53, 67
NGS_96_F_A8	AATGATACGGCGACCACCGAGATCTACACTTGAGCCTACACTCTTTCCCTACACGACGCTCTTCC	53, 67
NGS_96_F_A9	AATGATACGGCGACCACCGAGATCTACACACACGATCACACTCTTTCCCTACACGACGCTCTTCC	53, 67
NGS_96_F_A10	AATGATACGGCGACCACCGAGATCTACACGGTCCAGAACACTCTTTCCCTACACGACGCTCTTCC	53, 67
NGS_96_F_A11	AATGATACGGCGACCACCGAGATCTACACGTATAACAACACTCTTTCCCTACACGACGCTCTTCC	53, 67
NGS_96_F_A12	AATGATACGGCGACCACCGAGATCTACACTTCGCTGAACACTCTTTCCCTACACGACGCTCTTCC	53, 67
NGS round 2 PCR reverse primers:
NGS_96_R_E1	CAAGCAGAAGACGGCATACGAGATCATGATCGGTGACTGGAGTTCAGACGTGTGCTCTTC	53, 67
NGS_96_R_E2	CAAGCAGAAGACGGCATACGAGATAGGATCTAGTGACTGGAGTTCAGACGTGTGCTCTTC	53, 67
NGS_96_R_E3	CAAGCAGAAGACGGCATACGAGATGACAGTAAGTGACTGGAGTTCAGACGTGTGCTCTTC	53, 67
NGS_96_R_E4	CAAGCAGAAGACGGCATACGAGATCCTATGCCGTGACTGGAGTTCAGACGTGTGCTCTTC	53, 67
NGS_96_R_E5	CAAGCAGAAGACGGCATACGAGATTCGCCTTGGTGACTGGAGTTCAGACGTGTGCTCTTC	53, 67
NGS_96_R_E6	CAAGCAGAAGACGGCATACGAGATATAGCGTCGTGACTGGAGTTCAGACGTGTGCTCTTC	53, 67
NGS_96_R_E7	CAAGCAGAAGACGGCATACGAGATGAAGAAGTGTGACTGGAGTTCAGACGTGTGCTCTTC	53, 67
NGS_96_R_E8	CAAGCAGAAGACGGCATACGAGATATTCTAGGGTGACTGGAGTTCAGACGTGTGCTCTTC	53, 67