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. Author manuscript; available in PMC: 2025 Dec 13.
Published in final edited form as: Nat Protoc. 2024 Dec 15;20(6):1546–1583. doi: 10.1038/s41596-024-01090-z
Component Amount per reaction (μL) Final concentration
NebNext PCR Master Mix (2x) 12.5 1x
Primer Payload-Gibson-F (Table 1) (50μM) 0.1 0.2μM
Primer Payload-Gibson-R (Table 1) (50μM) 0.1 0.2μM
Template (10ng/μL) 1
UltraPure water 11.3
Total volume 25