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. Author manuscript; available in PMC: 2025 Dec 14.
Published in final edited form as: J Proteome Res. 2025 Nov 10;24(12):6079–6090. doi: 10.1021/acs.jproteome.5c00626

Table 2.

Diagnostic Performance of Tetra- Antennary Markers at Site N241 for all HCC, Early HCC, and MASLDa

all HCC (n = 59) vs cirrhosis (N = 60)
early stage (N = 37) vs cirrhosis (N = 60)
MASLD (N = 23) vs cirrhosis (N = 60)
markers AUC 95% CI p-value AUC 95% CI p-value AUC 95% CI p-value
a. Diagnostic Performance of Individual Markers at Site N241 for Al HCC, Early HCC, and MASLD
AFP 0.755 (0.665,0.836) <0.0001 0.739 (0.632,0.838) 0.0001 0.848 (0.715,0.954) 0.0001
gender 0.572 (0.488,0.656) 0.1320 0.558 (0.456,0.650) 0.2948 0.617 (0.492,0.751) 0.0955
age 0.695 (0.595,0.782) 0.0003 0.738 (0.635,0.827) 0.0001 0.624 (0.45,0.792) 0.1759
A4G4F3S2 0.563 (0.461,0.663) 0.2360 0.552 (0.438,0.665) 0.3874 0.559 (0.384,0.728) 0.5174
A4G4F1S3 0.631 (0.522,0.731) 0.0132 0.601 (0.486,0.718) 0.0959 0.502 (0.335,0.684) 0.9903
A4G4F3S3 0.658 (0.559,0.753) 0.0030 0.667 (0.553,0.777) 0.0059 0.665 (0.506,0.826) 0.0660
A4G4F2S4 0.573 (0.472,0.674) 0.1593 0.582 (0.462,0.691) 0.1706 0.574 (0.404,0.748) 0.4065
b. Diagnostic Performance of Glycopeptide Markers at Site N241 in the Combination of AFP with All HCC, early HCC, and MASLD
AFP 0.755 (0.665,0.836) Reference 0.739 (0.632,0.838) Reference 0.848 (0.715,0.954) Reference
AFP + A4G4F3S2 0.796 (0.718,0.870) 0.5040 0.781 (0.678,0.875) 0.5658 0.841 (0.698,0.957) 0.9152
AFP + A4G4F1S3 0.763 (0.675,0.842) 0.9114 0.743 (0.633,0.845) 0.9602 0.845 (0.700,0.952) 0.9565
AFP + A4G4F3S3 0.766 (0.686,0.846) 0.8660 0.756 (0.652,0.857) 0.826 0.862 (0.720,0.961) 0.8890
AFP + A4G4F2S4 0.802 (0.723,0.877) 0.4358 0.789 (0.691,0.880) 0.4888 0.836 (0.693,0.945) 0.8732
c. Diagnostic Performance of Glycopeptide Markers at Site N241 in the Combination of Gender with All HCC, Early HCC, and MASLD
AFP 0.755 (0.665,0.836) Reference 0.739 (0.632,0.838) Reference 0.848 (0.715,0.954) Reference
Gender + A4G4F3S2 0.662 (0.555,0.755) 0.1592 0.634 (0.515,0.75) 0.1955 0.634 (0.461,0.812) 0.0439
Gender + A4G4F1S3 0.602 (0.495,0.709) 0.0217 0.598 (0.484,0.711) 0.076 0.708 (0.542,0.874) 0.1671
Gender + A4G4F3S3 0.614 (0.511,0.718) 0.0375 0.591 (0.475,0.708) 0.0707 0.684 (0.514,0.853) 0.1185
Gender + A4G4F2S4 0.717 (0.621,0.807) 0.543 0.712 (0.599,0.818) 0.7214 0.746 (0.587,0.889) 0.3009
d. Diagnostic Performance of Glycopeptide Markers at Site N241 in the Combination of Gender with All HCC, Early HCC, and MASLD
AFP 0.755 (0.665,0.836) Reference 0.739 (0.632,0.838) Reference 0.848 (0.715,0.954) Reference
Age + A4G4F3S2 0.718 (0.618,0.806) 0.5663 0.751 (0.651,0.841) 0.8743 0.621 (0.447,0.8) 0.0414
Age + A4G4F1S3 0.712 (0.616,0.805) 0.5073 0.755 (0.654,0.836) 0.8285 0.622 (0.447,0.797) 0.0408
Age + A4G4F3S3 0.704 (0.602,0.793) 0.4347 0.740 (0.641,0.831) 0.9925 0.626 (0.452,0.807) 0.0426
Age + A4G4F2S4 0.736 (0.631,0.823) 0.7641 0.781 (0.691,0.866) 0.5603 0.657 (0.493,0.816) 0.0795
a

p-values in Table 2a are based on Wilcoxon test. P-values in Table 2bd are based on the comparisons of AUC of the combination panels vs AFP only.