TABLE 2.

Grepafloxacin transport in EHBR intestinal tissue mounted in an Ussing chamber and inhibitory effects of P-gp and MRP2 modulators^a

Rat strain and treatment	Permeability coefficient (μl/cm2/min)		Net secretion (μl/cm2/min) [(s-m) − (m-s)]	Secretory ratio [(s-m)/(m-s)]
	m-s	s-m
SDR (control)	0.759 ± 0.100	3.25 ± 0.48	2.49 ± 0.40	4.32 ± 0.36
EHBR
None	0.652 ± 0.127	1.06 ± 0.17b	0.404 ± 0.195b	1.77 ± 0.37b
Cyclosporine (10 μM)	1.26 ± 0.28c	1.34 ± 0.28	0.087 ± 0.108	1.11 ± 0.11c
Probenecid (1 mM)	1.12 ± 0.13c	2.07 ± 0.33c	0.951 ± 0.429	2.04 ± 0.59
Cyclosporine (10 μM) + probenecid (1 mM)	1.68 ± 0.09c	1.19 ± 0.14	−0.485 ± 0.208c	0.727 ± 0.123c

^aThe time course of grepafloxacin (10 μM) transport across the intestinal tissue was evaluated. The modulators were added to the donor solution. The experimental solution was adjusted to pH 7.4, and the temperature was maintained at 37°C.

^bSignificantly different from the value for SDRs (control) by Student’s t test (P < 0.05).

^cSignificantly different from the value for EHBRs by Student’s t test (P < 0.05).