TABLE 3.

Grepafloxacin absorption from intestine by in situ loop method in SDRs and EHBRs and inhibitory effects of P-gp and MRP2 modulators^a

Strain and inhibitor	% Absorption
	Duodenum	Jejunum	Ileum
SDR
None	77.0 ± 3.6	41.8 ± 4.0	40.5 ± 4.3
Cyclosporine (5 μM)	69.7 ± 5.2	50.8 ± 4.6	59.2 ± 2.5b
Probenecid (1 mM)	57.2 ± 2.0b	44.2 ± 4.1	45.2 ± 1.4
EHBR, none	59.2 ± 6.2b	39.8 ± 8.1	45.8 ± 6.0

^aIn the loop absorption experiment, 0.5 ml of isotonic MES buffer containing 10 μM grepafloxacin (pH 6.0) was administered into a 10-cm loop of duodenum, jejunum, or ileum. The luminal fluid in each loop was collected 15 min after administration. The fraction absorbed was estimated as the difference between the dose administered and the amount remaining in the loops. Each datum represents the mean ± SE of four to six experiments.

^bSignificantly different from the corresponding value for SDRs by Student’s t test (P < 0.05).