TABLE 4.

Functional replacement of P. aeruginosa OprM outer membrane efflux protein by SmeC

P. aeruginosa strain	Relevent genotype	Plasmid	MIC (μg/ml) ofc:
			CAR	CPZ	CIP	NOR	CAM	ERY	NOV
ML5087	Wild type	None	64	8	0.4	1	128	256	128
K1112	nalB	None	512	32	1.6	4	512	512	>512
K1113	nalB ΔoprM	None	0.5	0.25	0.006	0.015	2	32	8
K1113	nalB ΔoprM	pRK415 (vector)	0.5	0.25	0.006	0.015	2	32	8
K1113	nalB ΔoprM	pLZ436 (pRK415::smeCa)	8	4	3.2	2	64	512	128
K1113	nalB ΔoprM	pLZ437 (pRK415::smeCb)	0.5	2	1.6	1	32	128	32

^asmeC was cloned in the same orientation as plac.

^bsmeC was cloned in the opposite orientation to plac.

^cAbbreviations: CAR, carbenicillin; CPZ, cefoperazone; CIP, ciprofloxacin; NOR, norfloxacin; CAM, chloramphenicol; ERY, erythromycin; NOV, novobiocin.