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. 1967 Mar;102(3):767–773. doi: 10.1042/bj1020767

Studies of the chemical composition of a healing skin wound in rats, and of the concentrations of some constituents of tissues distant from the healing wound

John Candlish 1, N Chandra 1
PMCID: PMC1270327  PMID: 16742493

Abstract

1. A skin lesion was made in rats by dorsal incision and the insertion of a polythene tube. 2. Over a period of 25 days after wounding, assays were performed for ascorbic acid, DNA, hydroxyproline, methionine, tryptophan, tyrosine and free amino acids in the lesion tissue. 3. The neutral-salt-soluble proteins of the lesion tissue were fractionated on DEAE-Sephadex, with the separation of fibrinogen and γ-globulin from a serum protein fraction. 4. Over a period of 20 days after wounding, in wounded rats and in controls, assays were conducted for: ascorbic acid in lens and liver, hydroxyproline, soluble protein, methionine and water in muscle and tendon, and free amino acids in muscle. 5. Relative to controls there was a decrease in lens and liver ascorbic acid, a rise in tendon hydroxyproline, a rise in muscle free amino acids, a fall in muscle protein and a rise in tendon and muscle water.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADAMSONS R. J., MUSCO F., ENQUIST I. F. THE RELATIONSHIP OF COLLAGEN CONTENT TO WOUND STRENGTH IN NORMAL AND SCORBUTIC ANIMALS. Surg Gynecol Obstet. 1964 Aug;119:323–329. [PubMed] [Google Scholar]
  2. CANDLISH J. K., TRISTRAM G. R. THE RESISTANCE TO DISPERSION OF COLLAGEN FIBRES FORMED IN VITRO IN THE PRESENCE OF ASCORBIC ACID. Biochim Biophys Acta. 1963 Oct 29;78:289–294. doi: 10.1016/0006-3002(63)91639-1. [DOI] [PubMed] [Google Scholar]
  3. CMUCHALOVA B., CHVAPIL M. Changes in deoxyribonucleic acid, collagen and ascorbic acid during the development of carrageenan granuloma. Biochim Biophys Acta. 1963 Mar 5;69:565–567. doi: 10.1016/0006-3002(63)91310-6. [DOI] [PubMed] [Google Scholar]
  4. Cuthbertson D. P. The disturbance of metabolism produced by bony and non-bony injury, with notes on certain abnormal conditions of bone. Biochem J. 1930;24(4):1244–1263. doi: 10.1042/bj0241244. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FROMM H. J., NORDLIE R. C. Experimental injury and sulfur amino acid metabolism. J Biol Chem. 1956 Dec;223(2):737–741. [PubMed] [Google Scholar]
  6. LEACH A. A. A study of the protein impurities in gelatins with ion-exchange resins. Biochem J. 1960 Jan;74:61–69. doi: 10.1042/bj0740061. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LEVENSON S. M., GEEVER E. F., CROWLEY L. V., OATES J. F., 3rd, BERARD C. W., ROSEN H. THE HEALING OF RAT SKIN WOUNDS. Ann Surg. 1965 Feb;161:293–308. doi: 10.1097/00000658-196502000-00019. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. NEUMAN R. E., LOGAN M. A. The determination of hydroxyproline. J Biol Chem. 1950 May;184(1):299–306. [PubMed] [Google Scholar]
  9. ROE J. H. Chemical determination of ascorbic, dehydroascorbic, and diketogulonic acids. Methods Biochem Anal. 1954;1:115–139. doi: 10.1002/9780470110171.ch5. [DOI] [PubMed] [Google Scholar]
  10. RYAN W. L. REGULATION OF THE FREE AMINO ACIDS OF SKIN BY HYDROCORTISONE. J Invest Dermatol. 1964 Aug;43:121–123. doi: 10.1038/jid.1964.128. [DOI] [PubMed] [Google Scholar]
  11. SCHILLING J. A., MILCH L. E. Fractional analysis of experimental wound fluid. Proc Soc Exp Biol Med. 1955 Jun;89(2):189–192. doi: 10.3181/00379727-89-21753. [DOI] [PubMed] [Google Scholar]
  12. TRNAVSKY K. EFFECT OF FORMALDEHYDE-INDUCED PERIARTHRITIS UPON THE COMPOSITION OF COTTON-PELLET GRANULOMA IN RATS. J Pharm Pharmacol. 1965 Apr;17:261–262. doi: 10.1111/j.2042-7158.1965.tb07665.x. [DOI] [PubMed] [Google Scholar]

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