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Benzimidazole nucleosides inhibited HCMV replication but not viral DNA synthesis. Selected concentrations of TCRB (▪) and 5′-dTCRB (▵) were incubated in duplicate wells with HFF cells infected with HCMV at 3 PFU/cell. Viral DNA was detected by DNA-DNA hybridization, and yield was quantified by serial dilution of supernatants across 96-well culture plates seeded with HFF cells.
