FIG. 6.

Inhibition of cleavage of concatemeric DNA to monomeric genomic units by benzimidazole nucleoside analogs. Selected concentrations of TCRB (▪), BDCRB (◊), 5′-dTCRB (▵), and 1263W94 (•) were incubated in duplicate wells with HFF cells infected with HCMV at 3 PFU/cell. After 3 days, the cells were suspended in agarose and digested to release DNA. The DNA was separated by contour-clamped homogeneous electric field separation, transferred to a nylon membrane, and probed for HCMV-specific sequences. The amount of genomic-length monomeric DNA is expressed as a percentage of the total amount of viral DNA in the well. In the absence of test compounds, monomeric DNA was 25% ± 5% of the total DNA in three determinations.