TABLE 2.
Mode-of-action comparisons among benzimidazole nucleosides
| Test compound | IC90a (μM) by:
|
IC50 (μM) for DNA synthesisd | |
|---|---|---|---|
| Maturation assayb | Yield assayc | ||
| BDCRB | 0.08 | 0.29 ± 0.15 | >25e |
| 5′-dBDCRB | <0.04e | 0.018 ± 0.003 | >5 |
| 5′-dTCRB | 0.03 | 0.36 ± 0.26 | >25 |
| 1263W94 | 27 | 0.55 ± 0.06 | 0.3 |
a
IC90, 90% inhibitory concentration.
b
The maturation assay was a single-cycle yield reduction experiment in which 20 μM BDCRB was removed from HCMV-infected cultures and replaced at 96 h postinfection by the test compound.
c
Data are from two or three single-cycle yield reduction experiments in which the test compound was present for the entire experiment.
d
The assay was a single-cycle yield experiment in which the amount of DNA was measured by a hybridization assay. IC50, 50% inhibitory concentration.
e
The 50 or 90% inhibitory concentration was not reached at the noted highest or lowest concentration tested.