Table 3.

Showing different PCR conditions used in the study

H. pylori PCR conditions
glmM gene	H. pylori specifc-16srRNA
An initial denaturation at 94 °C for 5 min	An initial denaturation at 94 °C for 5 min
35 cycles of denaturation at 94º C for 30 s	35 cycles of denaturation at 94º C for 30 s
Annealing at 57º C for 40 s	Annealing at 50º C for 30 s
Extension 72˚C for 40 s	Extension 72˚C 30 s
Final extension at 72º C for 10 min	Final extension 72˚C 7 min