FIG. 2.

Preinoculation treatment with adefovir does not prevent the initial viral CCC DNA formation in primary duck hepatocyte cultures. Two days after seeding, primary duck hepatocyte cultures were inoculated (day 0) with an infectious DHBV serum. Intracellular viral DNA was analyzed by Southern blotting and specific hybridization at the indicated time points during cell culture. Adefovir or lamivudine was added 1 day before inoculation (day −1), at the indicated concentrations, and for 11 consecutive days with a daily medium change. The viral replicative intermediates are indicated. Days are indicated above the lanes. (A) Untreated control culture. (B) Adefovir-treated culture. (C) Lamivudine-treated culture. Relative band intensities for total DHBV DNA and for CCC DHBV DNA were quantified by PhosphoImager scanning; the results are presented in arbitrary units. L DNA, linear DNA; SS DNA, single-stranded DNA.