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RP-HPLC analysis and chemical structures for KNI-272 and its oxidized forms generated by treatment with hydrogen peroxide. (Top) RP-HPLC tracing of KNI-272 after treatment with 10 mM H2O2. KNI-272 and the oxidized products, P1 and P2, are indicated in the RP-HPLC tracing. Structures: A, KNI-272; B, KNI-272 oxidized at the sulfur in the S-methyl cysteine moiety; C, KNI-272 oxidized on the sulfur of the S-methyl cysteine residue and on the sulfur in the thioproline ring.
