Table 3:

Mixed Model Results for Longitudinal Orexin Peptides

Percent of Baseline (23:00-11:00)
OXA (RIA)
Factor	Pairwise Comparison	Mean Difference	F (df)	p-value
Intervention	SD vs. C	+8.22%	7.3 (1,32)	0.011
Time			1.15 (5,32)	0.357
Intervention x Time			1.6 (5,32)	0.181
Prepro-Orexin (MS)
Factor	Pairwise Comparison	Mean Difference	F (df)	p-value
Intervention	SD vs. C	+23.97%	22.5 (1,32)	<0.001
Time			0.53 (5,32)	0.755
Intervention x Time			0.3 (5,32)	0.907
Long OXA with Intact N-Terminal (MS)
Factor	Pairwise Comparison	Mean Difference	F (df)	p-value
Intervention	SD vs. C	+38.7%	22.1 (1,32)	<0.001
Time			3.3 (5,32)	0.017
Intervention x Time			0.16 (5,32)	0.976
Short OXA with Intact N-Terminal (MS)
Factor	Pairwise Comparison	Mean Difference	F (df)	p-value
Intervention	SD vs. C	+33.6%	14.4 (1,33)	<0.001
Time			1.4 (5,33)	0.263
Intervention x Time			1.6 (5, 33)	0.200
Long OXB with Truncated N-Terminal (MS)
Factor	Pairwise Comparison	Mean Difference	F (df)	p-value
Intervention	SD vs. C	+21.12%	8.4 (1,32)	0.007
Time			1.5 (5,32)	0.220
Intervention x Time			0.58 (5,32)	0.718
Short OXB with Truncated N-Terminal (MS)
Factor	Pairwise Comparison	Mean Difference	F (df)	p-value
Intervention	SD vs. C	+29.35%	35.9 (1,32)	<0.001
Time			2.2 (5,32)	0.085
Intervention x Time			0.4 (5,32)	0.846
Orexin Stable Isotope Labeling Kinetics and Newly-Generated Orexin Peptides (07:00-19:00)
Long OXB (Normalized TTR%)
Factor	Pairwise Comparison	Mean Difference	F (df)	p-value
Intervention	SD vs. C	+0.014%	8.174 (1,14)	0.013
Time			19.05 (3,14)	<0.001
Intervention x Time			0.876 (3,14)	0.477
Newly-Generated Long OXB with Truncated N-Terminal (MS)
Intervention	SD vs. C	+0.967 pg/ml	34.61 (1,14)	<0.001
Time			20.62 (3,14)	<0.001
Intervention x Time			2.786 (3,14)	0.080

OXA: orexin-A; OXB: orexin-B; SD: sleep deprived; C: control; MS: mass spectrometry; TTR: tracer-tracee ratio; RIA: radioimmunoassay; pg: picogram; ml: milliliter