Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1967 Aug;104(2):534–536.3. doi: 10.1042/bj1040534

Purification and amino acid composition of monomeric and polymeric collagens

F S Steven 1, D S Jackson 1
PMCID: PMC1270616  PMID: 6048794

Abstract

The preparations and amino acid compositions of highly purified tropocollagen and insoluble polymerized collagen are described. These collagens appear to be very suitable for comparative studies in an investigation of the cross-linkages that are introduced into tropocollagen during the formation of polymerized collagen.

Full text

PDF
534

Images in this article

536-1Plate 1
on p.536-1
536-2Plate 2
on p.536-2
536-3Plate 3
on p.536-3

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BORNSTEIN P., PIEZ K. A. COLLAGEN: STRUCTURAL STUDIES BASED ON THE CLEAVAGE OF METHIONYL BONDS. Science. 1965 Jun 4;148(3675):1353–1355. doi: 10.1126/science.148.3675.1353. [DOI] [PubMed] [Google Scholar]
  2. Blumenfeld O. O., Gallop P. M. Amino aldehydes in tropocollagen: the nature of a probable cross-link. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1260–1267. doi: 10.1073/pnas.56.4.1260. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Drake M. P., Davison P. F., Bump S., Schmitt F. O. Action of proteolytic enzymes on tropocollagen and insoluble collagen. Biochemistry. 1966 Jan;5(1):301–312. doi: 10.1021/bi00865a039. [DOI] [PubMed] [Google Scholar]
  4. EASTOE J. E. The amino acid composition of mammalian collagen and gelatin. Biochem J. 1955 Dec;61(4):589–600. doi: 10.1042/bj0610589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FLEISCHMAJER R., FISHMAN L. AMINO-ACID COMPOSITION OF HUMAN DERMAL COLLAGEN. Nature. 1965 Jan 16;205:264–266. doi: 10.1038/205264a0. [DOI] [PubMed] [Google Scholar]
  6. GALLOP P. M. Particle size and shape in a citrate extract of ichthyocol. Arch Biochem Biophys. 1955 Feb;54(2):486–500. doi: 10.1016/0003-9861(55)90061-8. [DOI] [PubMed] [Google Scholar]
  7. JACKSON D. S., LEACH A. A., JACOBS S. The amino acid composition of the collagen fractions of rabbit skin. Biochim Biophys Acta. 1958 Feb;27(2):418–420. doi: 10.1016/0006-3002(58)90356-1. [DOI] [PubMed] [Google Scholar]
  8. PIEZ K. A., LEWIS M. S., MARTIN G. R., GROSS J. Subunits of the collagen molecule. Biochim Biophys Acta. 1961 Nov 11;53:596–598. doi: 10.1016/0006-3002(61)90226-8. [DOI] [PubMed] [Google Scholar]
  9. PIEZ K. A., MORRIS L. A modified procedure for the automatic analysis of amino acids. Anal Biochem. 1960 Nov;1:187–201. doi: 10.1016/0003-2697(60)90045-2. [DOI] [PubMed] [Google Scholar]
  10. STEVEN F. S. THE NISHIHARA TECHNIQUE FOR THE SOLUBILIZATION OF COLLAGEN. APPLICATION TO THE PREPARATION OF SOLUBLE COLLAGENS FROM NORMAL AND RHEUMATOID CONNECTIVE TISSUE. Ann Rheum Dis. 1964 Jul;23:300–301. doi: 10.1136/ard.23.4.300. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. STEVEN F. S., TRISTRAM G. R. The presence of non-protein nitrogen in acetic acid-soluble calf-skin collagen. Biochem J. 1962 May;83:240–244. doi: 10.1042/bj0830240. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Veis A., Anesey J. Modes of intermolecular cross-linking in mature insoluble collagen. J Biol Chem. 1965 Oct;240(10):3899–3908. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES