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Detection and isolation of the antimicrobial substance. (A) TLC-bioautography of antimicrobial substance. A BuOH extract from cells of strain 168 (lane a) or strain JJ25 (lane b) was subjected to TLC. The arrow indicates the bioactive position. (B) HPLC profile of the crude antimicrobial substance. The arrow indicates the bioactive fraction. Details of the procedures for TLC bioautography and HPLC are described in Materials and Methods.
