Skip to main content
. 2025 Dec 3;16:1714617. doi: 10.3389/fpls.2025.1714617

Table 2.

Biofortification of Se in horticultural crops by soil/root zone application - recent reports.

Sr. No. Plant species/part Se compound applied Se concentration applied Mode of application Physiological effects/ Se biofortification Fold change (compared to control) References
1 Chives
(Allium schoenoprasum)
Na2SeO3 0, 20, 40, 80, and 160 mg L−1 Soil application
(three times)
- Regulation of phenylpropanoid pathway
- 40.2 mg kg-1 DW Se accumulation in response 160 mg L-1 application
2010-fold Chen et al., 2025
2 Citrus
(Citrus reticulata)
Na2SeO3 0 and 100 mg L-1 Soil application - 9.25 ng g-1 Se fruit
- 92.1 ng g-1 Se in leaf
2.74-fold
1.63-fold
Wang et al., 2024
3 Onion
(Allium cepa L.)
Na2SeO4 0, 10, 25, 50, 100
and 200 g ha-1
Soil application - Increase in onion yield up to 99 kg ha-1
- 0.600 mg kg-1 Se in bulb
60-fold Machado et al., 2024
4 Komatsuna
(Brassica rapa var. perviridis)
2-iminoselenazolidin-4-ones (ISeA) 0 and 10 mg L-1 Root zone - 34.7 mg kg-1 Se in leaves 35-fold Semenova et al., 2024
5 Chard
(Beta vulgaris subsp. cicla)
2-iminoselenazolidin-4-ones (ISeA) 0 and 10 mg L-1 Root zone - 23.8 mg kg-1 Se in leaves
- 55.6 mg kg-1 Se in petioles
24-fold
56-fold
Semenova et al., 2024
6 Sweet cherry
(Prunus avium)
Na2SeO4 250 and 500 g ha−1 Soil application - Leaf Se concentrations up to 1.178 mg kg-1
(two-year average)
- Fruit Se up to 2.45 µg 100 g-1 FW (two-year average)
- No leaf or fruit injury by Se application
27.3-fold
8.16-fold
Wójcik, 2024
7 Tomato
(Lycopersicum esculentum L.)
Na2SeO4
Na2SeO3
0, 1 & 5 mg kg-1 each Soil application - Selenate increased tomato yield and Se accumulation in tomato root (28.5 mg kg-1), stem (16 mg kg-1), leaves (34 mg kg-1) and in fruits.
- Selenite caused Se accumulation in tomato root (7.5 mg kg-1), stem (2 mg kg-1), leaves (6.2 mg kg-1) and in fruits.
18.5-fold (root)
11.3-fold (stem)
28.6-fold (leaf)
12.3-fold (root)
1.33-fold (stem)
4.13-fold (leaf)
Kang et al., 2024
8 Cowpea
(Vigna unguiculata)
Sodium selenate 0, 10, 25, and 50 g Se ha−1 combined with S (S (0, 15, 30, and 60 g ha−1) Soil application - In the absence of S, leaf Se concentrations went up to 2.55 mg kg-1 (average two-year)
- A two-year average seed Se contents were 1.45 mg kg-1
8.5-fold change
4.83-fold change
Silva et al., 2023
9 Rocket
(Eruca sativa)
Na2SeO4 0, 2.6, 3.9, or 5.2 μmol L−1 Root zone/Hydroponics - Leaf Se contents up to 55 µg g-1 DW in response to 5.2 μmol L−1 110-fold change Francini et al., 2023
10 Lettuce (Lactuca sativa) - Leaf Se contents up to 15 µg g-1 DW in response to 5.2 μmol L−1 30-fold change Francini et al., 2023
11 Spinach
(Spinacia oleracea)
- Leaf Se contents up to 15 µg g-1 DW in response to 5.2 μmol L−1 30-fold change Francini et al., 2023
12 Wheat
(Triticum aestivum L.)
Sodium selenite and sodium selenate 0, 10 µM and mixture of both Root zone/Hydroponics - Grain Se contents were 150 mg kg-1 in response to selenite treatment 300-fold change Subirana et al., 2023
13 Psyllium (Plantago ovata) sodium selenate 0, 10, 50, 100 and 500 µM Agar medium/Root zone - Highest accumulation of 457.65 µg g-1 FW at 500 µM Se treatment ~250-fold change Dey and Raychaudhuri, 2024