FIG. 1.
Effects of 14-member macrolides and DEX on neutrophil apoptosis, as estimated by morphology (A) and annexin V binding (B), in the presence (solid bars) or absence (open bars) of LPS. Separated neutrophils were incubated with a 14-member macrolide (10 μg/ml) or DEX (10−7 M; 43 ng/ml), and LPS (1 μg/ml) was then added to the cell suspension. After a 24-h incubation, neutrophils were recovered and analyzed for apoptosis. The control (CTRL) contained 0.1% DMSO in IMDM. Each value is the mean ± standard deviation from three determinations. ∗∗, P < 0.01 (compared with the control in the absence of LPS).