FIG. 2.

Effects of 14-member macrolides, DEX, or MG-132 (10⁻⁶ M) on IL-8 production by human neutrophils in the presence of LPS after 24 (A) or 48 (B) h of incubation. Separated neutrophils were incubated with each reagent at the indicated concentrations, and LPS (1 μg/ml) was then added to the cell suspension. After a 24- or 48-h incubation, IL-8 levels in culture supernatants were determined. The control (CTRL) contained 0.1% DMSO in IMDM. Each value is the mean ± standard deviation from three determinations. ∗∗, P < 0.01; ∗, P < 0.05 (compared with the control in the presence of LPS).