FIG. 3.

Effects of EMA (10 μg/ml), CAM (10 μg/ml), and DEX (10⁻⁷ M; 43 ng/ml) on IL-8 gene expression in LPS-stimulated human neutrophils. IL-8 gene expression of uncultured neutrophils without LPS stimulation was also examined as a negative control. The control medium (CTRL) contained 0.1% DMSO in IMDM. Results shown are IL-8 mRNA transcript levels (upper panel) and control G3PDH mRNA transcript levels (lower panel) in human neutrophils. Bars in graph represent densitometric units obtained from an autoradiogram by Northern blot analysis and expressed as the ratios of IL-8 mRNA transcript levels to G3PDH mRNA transcript levels.