Figure 6.

Knock down of TDP-43 causes the rescue of exon 3 inclusion. (A) Upper panel, western blot analysis of the cells treated with the siRNA oligonucleotide against TDP-43 (siRNA TDP43), control siRNA and mock transfected (lanes 1–3, respectively). TDP-43 expression was probed by western blot using a polyclonal antiserum. Lower panel, normalization control western blot with antibody against α-tubulin. (B) Shows splicing pattern analysis by ethidium bromide staining a 2% agarose gel of RT–PCR products after cotransfection of pApo-wt, pApo-A97T and pApo-ISE3m along with either the siRNA TDP43 (lanes 1, 4 and 7) or a control siRNA oligonucleotides (lanes 2, 5 and 8). The transfection of different variants of pApo gene system with no siRNA oligonucleotide was included as a control (lanes 3, 6 and 9).