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Effect of DNA topoisomerase inhibitors on DEPC reactivity toward the 176 bp DNA fragment. Digestion products were resolved on 8% polyacrylamide gel containing 7 M urea. Control tracks contained no drug. Numbers on the left side of the gel refer to the standard numbering scheme for the nucleotide sequence of the DNA fragment based on comparison to the position of the guanines on guanine-specific track.
