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. 2005 Oct 27;33(18):5965–5977. doi: 10.1093/nar/gki905

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© The Author 2005. Published by Oxford University Press. All rights reserved

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Effect of overexpression of r-proteins on the rpL3 splicing pattern. (A) Analysis of rpL3 expression in control cells (PC12) and in clones stably transfected with HA-tagged rpL3 cDNA (L3–8 and L3–9) or with HA-tagged rpL12 cDNA (L12). Northern blot hybridizations of total RNA were performed with the indicated probes. Protein samples purified from the same clones were analyzed by western blotting with an HA-tag specific antibody (αHA). (B) Quantification of mRNAs shown in panel A. The amounts of HA-mRNA are expressed in arbitrary units after normalization to GAPDH mRNA levels. The mRNA levels of endogenous isoforms were normalized to GAPDH and expressed as a ratio to endogenous levels in untreated control cells. (+), addition of doxycycline; (−), removal of doxycycline. The average of three independent experiments is shown, with SDs.