Table 6.

The physical properties and stability test results for five biological RNA sequences

Sequences		RNA	NCBI accession	$\Delta G_{37}^{\text{o}}$ (kcal/mol)	Tm (°C)	$T_{\text{m}}-\Delta G_{37}^{\text{o}}$ test	V	$V-\Delta G_{37}^{\text{o}}$ test
B1	5′-GGAUACGGCC AUACUGCGCA GAAAGCACCG CUUCCCAUCC GAACAGCGAA GUUAAGCUGC GCCAGGCGGU GUUAGUACUG GGGUGGGCGA CCACCCGGGA AUCCACCGUG CCGUAUCCU	5S RNA	M34003	−61.78	93.6	+	4.13	+
B2	5′-GGUCCUAUGG UCUAGUGGUC AGGACAUUGG ACUCUGAAUC CAGUAACCCG AGUUCAAAUC UCGGUAGGAC CU	Gln tRNA		−29.65	87.4	+	2.65	+
B3	5′-AGCACCGCCU GCUACUUACA UCGCAUUUAU CUUUCGCCUU UUACUAAAGA UAGCCGUGAG UGAGCAGGCA CUGCGGUGCA UUGACCCAAU UUUUGGAGCC CCCUCAAAAG GGCA	U5 RNA	AF095839	−42.96	86.8	+	7.25	+
B4	5′-UGCAGUUUGC UGCGCUAUUA GUUUGGAACA ACACUGAGAA GAUUAGCAUG GCCCCUGCGC AAGGACGGCA UCUUUCUUUG AGAGGUGUGC UGGGCUCGCC CAGCUUUU	U6 RNA	AF053588	−38.43	67.4	+	18.06	−
B5	5′-GACGUGUUAC AGCUCUUUUA GAAUUUGUCU AGUAGGCUUU CUGGCUUUUC ACCGGAAAGC CCCU	U7 RNA	M17910	−19.45	93.4	+	8.85	+

$\Delta G_{37}^{\text{o}}$, T_m and V denote the free energy, melting temperature and Valley index, respectively. The $T_{\text{m}}-\Delta G_{37}^{\text{o}}$ and $V-\Delta G_{37}^{\text{o}}$ test results (+ or −) at 90% confidence level are calculated using the thermodynamic scatter plots (see text and Figure 4).