Table 2.

Summary of genes tagged by Gene CATCHR: tagging efficiencies, expression patterns generated and comparisons with previously reported data

^a,b(number of positive colonies)/(total number of colonies tested).

^a6–99 colonies tested (mean = 21).

^b3–78 colonies tested (mean = 15).

^cAll expression patterns were examined in hermaphrodites, except where noted. Symbols used to describe GFP expression patterns generated from Gene CATCHR transgenes: E, embryo; L1–L4, larval stages 1 through 4; A, adult; ND, not done; plus, GFP detected; minus, GFP not detected; asterisks, GFP detected, expression from reporter transgene previously unpublished; shaded cells, protein localization data previously unpublished.

^dAs described in WormBase (http://www.wormbase.org) and references therein or the Nematode Expression Pattern DataBase (NEXTDB, http://nematode.lab.nig.ac.jp) with additional information as indicated. 1, translational reporter-fusion; 2, RNA in situ hybridization; 3, northern analysis/dot blot; 4, antibody staining; 5, transcriptional reporter-fusion.

^eExpression patterns from a translational fusion have been analyzed in adults, but it is unclear what other developmental stages were examined (44).

^fConstructs containing a reassembled GFP tag were detected by PCR; however, the constructs were not successfully isolated from yeast.

^gExpression examined in males.

^hA. Puoti, personal communication.

ⁱAfter cytoduction, no colonies formed on SC-HIS plus 5-FOA plates.

^jAlso K. Nakayama, personal communication.

^kM. Schvarzstein and A. M. Spence, manuscript in preparation.