TABLE 3.
Comparison of wt and YMDD-mutant polymerases based on IC50s for lamivudine and on Ki values for lamivudine triphosphatea
| Polymerase | Lamivudineb
|
Lamivudine Triphosphatec
|
||
|---|---|---|---|---|
| IC50 (μM) | Fold increase relative to wt | Ki (μM) | Fold increase relative to wt | |
| wt | 0.049 | NA | 0.032 | NA |
| LMMV | >200 | >10,000 | 37 | 1,100 |
| MI | >200 | >10,000 | 31 | 970 |
| LMMI | >200 | >10,000 | 43 | 1,300 |
| LM | 0.9 | 18 | 0.255 | 8 |
| MV | 7.5 | 153 | 1.8 | 55 |
a
Abbreviations: LMMV, L528M M552V; MI, M552I; LMMI, L528M M552I; LM, L528M; MV, M552V; NA, not applicable.
b
Data from Allen et al. (1).
c
Data shown are averages of three assays. Standard error of the mean is <10%.