TABLE 5.
Catalytic specificities (kcat/Km) for wt and YMDD-mutant polymerases, determined by core polymerase assays using one labeled dNTP at Km and unlabeled dNTPs at 35 μM
| HBV polymerasea |
kcat/Km valuesb for each dNTP (turnover rate/μM)
|
|||
|---|---|---|---|---|
| dATP | dGTP | dCTP | TTP | |
| wt | 6.6 | 13.3 | 10.9 | 9.8 |
| LMMV | 3.0 | 7.0 | 7.7 | 4.1 |
| MI | 1.5 | 2.3 | 3.8 | 1.9 |
| LMMI | 2.6 | 8.5 | 6.9 | 6.0 |
| LM | 5.4 | 10.5 | 9.1 | 7.3 |
| MV | 1.6 | 3.2 | 3.7 | 2.4 |
a
Abbreviations: LMMV, L528M M552V; MI, M552I; LMMI, L528M M552I; LM, L528M; MV, M552V.
b
Values based on the assumption that the number of active DNA polymerase molecules is represented by the number of (−) strands that have completed synthesis to at least 200 nt.