TABLE 8.
Activities of wt and YMDD-mutant HBV polymerases at 4.4 μM dCTP and limiting concentrations of dGTP, dATP, and TTPa
| Concn of dATP, dGTP, and TTP | % of HBV polymerase activity
|
||||
|---|---|---|---|---|---|
| Actual (μM) | Fold (relative to measured cellular dNTP levels) | wt | LMMV | MI | LMMI |
| 16.4, 8.0, 18.4 | 4 | 101.9 | 91.5 | 80.6 | 93.1 |
| 4.1, 2.0, 4.6 | 1 | 93.3 | 66.0 | 46.0 | 67.7 |
| 2.0, 1.0, 2.3 | 0.5 | 85.6 | 49.2 | 30.9 | 50.3 |
| 0.41, 0.2, 0.46 | 0.1 | 62.0 | 23.4 | 12.7 | 23.6 |
a
Enzymatic activities were determined as the mean values of three measurements and expressed relative to calculated Vmax values at nonlimiting dNTP concentrations. Abbreviations: LMMV, L528M M552V; MI, M552I; LMMI, L528M M552I.