Fig. 2.
The mex-3 3′UTR is essential during temperature stress. (A) Violin plots of the total brood for control and mex-3 3′UTR deletion strains. Blue, gray and orange violins correspond to 15, 20 and 25°C growth, respectively. (B) Box and whisker plots depicting the hatch rate of embryos from A. Whiskers indicate the full range of the data; the box represents the range of the inner quartiles; the notch represents the median; each dot represents the hatch rate of the total brood produced from an adult hermaphrodite. Asterisks indicate outliers, as defined by the Tukey method. (C-F) Brood size and hatch rate data for infectious stress (C,D) and osmotic stress (E,F). In C,D, orange indicates Pseudomonas aeruginosa (PA14) while gray indicates E. coli (OP50) food. In E,F, orange indicates high salt (300 mM NaCl) while gray indicates standard salt (50 mM NaCl). (G) Time course to assess reversibility of the temperature-sensitive embryonic lethality phenotype. (H) Box and whiskers plot of the hatch rate post-recovery in each genotype. In all panels, gray lines indicate statistical significance within a genotype and red brackets indicate the same between genotypes in a one-way ANOVA with Bonferroni correction (Padj<0.05). All sample sizes and statistical test outcomes are listed in Table S3.