Fig. 3.

Elevated temperature decreases GFP::MEX-3 in maturing oocytes and embryos. (A) DIC and GFP images of the control and mex-3 3′UTR deletion mutant strains. The dashed white line outlines a gonad, green asterisks mark the syncytial region. The yellow and blue brackets mark developing oocytes. The −4 to −1 oocytes are labeled. (B) Box and whisker plots of oocyte fluorescence at standard and elevated temperature. Statistical significance is indicated as in Fig. 2. (C) Still frames from embryogenesis movies at t=0. The colored circles correspond to the markers in D. (D) Quantitation of GFP::MEX-3 in embryogenesis movies. The markers indicate the mean per time point of multiple movies per sample (n=2-5). The error bars are s.e.m., and the curves are fit to a sigmoidal equation to determine the maximal fluorescence, the half-time, and the rate of loss of the steady state GFP abundance, which incorporates both synthesis and decay. All sample sizes and statistical test outcomes are listed in Table S3.