表 1.

构建质粒所用引物序列

Nucleotide sequences of primers used in plasmid construct

Gene	Primer
LASS2, homo sapiens longevity assurance homolog 2 of yeast LAG1.
LASS2	Forward: 5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTTAATGCTCCAGACCTTGTATGATTA-3′
	Reverse: 5′-GGGGACCACTTTGTACAAGAAAGCTGGGTAGTCATTCTTACGATGGTTGT-3′
LASS2-T332A	Forward: 5′-TACCAGCTTTCCAGCTATGAACTTGTGGGCCATG-3′
	Reverse: 5′-CATGGCCCACAAGTTCATAGCTGGAAAGCTGGTA-3′
LASS2-S341A	Forward: 5′-TCTGTTTCTTCCCGGTCAGCGCGTTCATCTTCTACCAG-3′
	Reverse: 5′-CTGGTAGAAGATGAACGCGCTGACCGGGAAGAAACAGA-3′
LASS2-T346A	Forward: 5′-CTCTGAGCTCTCTGCTTCTTCCCGGTCACTGC-3′
	Reverse: 5′-GCAGTGACCGGGAAGAAGCAGAGAGCTCAGAG-3′
LASS2-S348A	Forward: 5′-TCCTCCCCCTCTGAGGCCTCTGTTTCTTCCCG-3′
	Reverse: 5′-CGGGAAGAAACAGAGGCCTCAGAGGGGGAGGA-3′
LASS2-S349A	Forward: 5′-CCTCCTCCCCCTCTGCGCTCTCTGTTTCTTC-3′
	Reverse: 5′-GAAGAAACAGAGAGCGCAGAGGGGGAGGAGG-3′