Table 2.

Representative type II glycosyltransferases and glycosidases whose cytoplasmic, transmembrane, and stem (CTS) domains serve as targeting signals for specific subcellular localization of heterologous enzymes. UniProt accession numbers and amino acid ranges defining the CTS or CT domains are indicated.

Subcellular targeting	Type II transmembrane protein	Acc. UniProtKB	CTS length (aa)
ER	Arabidopsis thaliana α-glucosidase I (GCSI)	F4HTM3	1-90
ER/cis-Golgi	Arabidopsis thaliana Golgi α1,2-mannosidase A (MNS1A)	Q9C512	1-88
	Arabidopsis thaliana N-acetylglucosaminyltransferase I (GNTI)	Q9XGM8	1-77
cis/medial-Golgi	Arabidopsis thaliana Golgi α1,2-mannosidase C (MNS1C)	Q93Y37	1-109
	Arabidopsis thaliana Golgi mannosidase II (GMII)	Q9LFR0	1-92
	Arabidopsis thaliana β1,2 xylosyltransferase (XYLT)	Q9LDH0	1-90
medial-Golgi	Arabidopsis thaliana α1,3-fucosyltransferase (FUT11)	Q9LJK1	1-66
	A. thaliana β1,2-galactosyltransferase (MUR3)	Q7XJ98	1-120
	Schistosoma mansoni α1,3-fucosyltransferase (FucTC)	E2EAI6	1-35 (CT)
Medial/trans-Golgi	Arabidopsis thaliana β1,2-N-acetylglucosaminyltransferase II (GNTII)	Q9FT88	1-76
	Homo sapiens β1,4-galactosyltransferase (B4GALT1)	P15291	1-68
	Arabidopsis thaliana Xyloglucan β1,2 galactosyltransferase (MUR3)	Q7XJ98	1-120
	Homo sapiens α1,6-fucosyltransferase (FUT8)	Q9BYC5	1-108
trans-Golgi	Homo sapiens α1,4-fucosyltransferase (FUT9)	Q9Y231	1-60
	Arabidopsis thaliana α1,3-galactosyltransferase (GALT1)	Q8L7F9	1-60
	Arabidopsis thaliana α1,4-fucosyltransferase (FUT13)	Q9C8W3	1-52
	Physcomitrium patens α1,4-fucosyltransferase (FT4)	A9T1L4	1-130
	Rattus norvegicus α2,6-sialyltransferase (ST6GAL1)	P13721	1-52
	Homo sapiens α2,3-sialyltransferase (ST3GAL4)	Q11206	1-116
	Homo sapiens α2,8 sialyltransferase (ST8SIA2)	Q92186	1-50
Plasma membrane	Arabidopsis thaliana β-hexosaminidase 3 (HEXO 3)	Q8L7S6	1-26 (CT)