Fig 2.

Catalytically inactive SpoVD requires its SEDS partner, SpoVE, to facilitate asymmetric division. (a and b) Quantification of the cytological profiling of cells sampled from sporulation-inducing plates after 20–22 h of growth. White circles indicate data from each replicate; bars indicate average means; and error bars indicate standard deviation. More than 1,000 total cells and over 100 visibly sporulating cells were analyzed per sample from a minimum of three biological replicates. (a) Distribution of visibly sporulating cells among the indicated stages of sporulation. See Fig. 1 for the distribution of WT cells. (b) Proportion of cells that complete and progress beyond asymmetric division, i.e., all visibly sporulating cells, as a percentage of the total cells profiled. Note that the data are normalized to WT (dotted line) and that the spoVD^S311A data were derived from Fig. 1. ****P < 0.0001. Statistical significance was determined using one-way ANOVA and Tukey’s test. (c) Western blot analyses of SpoVD levels in the indicated strains 14 h after growth on sporulation-inducing plates. The anti-Spo0A antibody was used as a proxy for measuring sporulation induction.