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. Author manuscript; available in PMC: 2025 Dec 19.
Published in final edited form as: J Am Chem Soc. 2025 Oct 23;147(44):40652–40663. doi: 10.1021/jacs.5c13262

Figure 4.

Figure 4.

(A) 5-week biofilm cultures of wild-type Mtb and Δrv0101 Mtb. The latter biofilm was fragile, thin, and absent in the reticular pattern. (B) Chemical complementation of M. tuberculosis Δrv0101 strain with kupyaphores. Growth curves for WT and Δrv0101 deletion mutants complemented with or without 200 nM 1a or 1c measured by the optical density at 600 nm (OD600) in Chelex-processed Sauton’s medium supplemented with 0.1 μM ZnII. (C) The extracted ion chromatograms of the natural and synthetic C18 kupyaphore 1c at m/z 848.6987 (ΔM ± 5 ppm) on LC–MS (ESI+).