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. Author manuscript; available in PMC: 2025 Dec 19.
Published in final edited form as: J Am Chem Soc. 2025 Oct 23;147(44):40652–40663. doi: 10.1021/jacs.5c13262

Figure 7.

Figure 7.

(A) Schematic of liposome experiments. High-affinity membrane impermeant Fluozin-3 salt surrogates ZnII deposit (e.g., ZnII demanding proteins in nascent Mtb). Extravesicular zinc was 20 μM. (B) Time-course change of normalized fluorescence intensity. Sodium lauryl sulfate and additional ZnBr2 were added at 90 min to lyse liposome and to secure Fmax. (C) Intravesicular zinc concentrations vs time. (D) Epifluorescence images showing the significant turn-on of intravesicular Fluozin-3 after adding kupyaphores and the integrity of liposome before lysis. Scale bars are 50 μm. (E) Apparent initial rates of zinc transport vs kupyaphore concentrations. (F) Proposed machinery of kupyaphores facilitating ZnII traversal across outer and inner Mtb membranes.