Table 1.

PCR Amplification of COL17A1 Genomic DNA

	Primer(5′→3′)
Position and PCRa	Forward	Reverse	Product Size(bp)
Exon 18, 1706delA:
1	taggagcgagtgtatggaag	ggatgatggtactcacagca	385
2	agaggatcaggaggagcata	cttcctcacgaacatccaga	155
Intron 19, 1822+14T/C:
1	agatgtgaagctgactcagg	agtcattcagctcttcatcc	187
2	agatgtgaagctgactcagg	aactcacttccaactgcagg	125
Intron 28, 2268+120T/C:
1	acaggagttcacggatacag	ctccttatcctccactccag	327
2	cggatacagtgagaactacc	ctccttatcctccactccag	316
Exon 37, 2700T/C:
1	agaggtgagaagtgtggcag	gcagaagagagcaaggaaga	263
2	tgagaagtgtggcagagagc	gtgcattgaatctcctgtcc	180
Exon 43, 2988A/C:
1	tacagggctgttagtgggtg	acttgctgcatctcttcagg	345
2	gctgactgcactgtgtgtgt	tcaccaccttctcactgctc	138
Exon 51, 3781C→T:
1	tttctctcctcccatcaccc	tgtccctttaagtgcctccc	374
2	ccagcctcatcccctccttt	ctccggaagctgtcgctgtt	236
Exon 52, 4214A/G:
1	caagtctttctctccaccga	ccacaaacaagaaagccagt	527
2	ctcctacagctcttccatga	gctgcatgctctctgacacc	242
Exon 54, 4424-5insC:
1	ttgatgtcccaagcttccag	ctaatgagcgtcagccttgc	419
2	tcaagactctaagggccagt	tggagcagtcaacacttacc	352

^aThe numbers 1 and 2 refer to the first PCR and the second/nested PCR, respectively.