Table A1.

Primers and PCR Conditions for the Sequence Analysis of VDR

Region and Primer Name	Primer Sequence(5′→3′)	MgCl2(nM)	Annealing Temperature(°C)	Size of PCR Product(bp)	GenBanka Accession Number
Promoter region:
1f-23h For	GAACCAGGATCATGTTTTGGA
1f-23h Rev	ACAATCCAAGTCTGTGGATAGATG	1.5b	57	655	BV210414
1f-2kb For	CAAGTTTTGCCAGATTCACC
1f-2kb Rev	CCGCAGCACTATACAGAGGT	1.5b	58	932	BV210415
1f-1kb For	CAAGTCCATATCTCCTTCTCCAG
1f-1kb Rev	AAGTGGTTTCCCTACCATCAAT	1.5b	59	855	BV210416
1f-4h For	AGATACCTCTGGTTCCTCTAATGC
1f-4h Rev	CACACTTGTTCACCTCCACAC	1.5b	59	631	BV210417
VDR1a-40h For	CTCAAGCATAGTGGCATGATCA
VDR1a-40h Rev	TTCCTGATACAAAAGATGTTCTACAATG	1.5	60	549	BV210418
VDR Cdx-2 G For	AGGATAGAGAAAATAATAGAAAACATT
VDR Cdx-2 A Rev	ACGTTAAGTTCAGAAAGATTAATTC	1.5	54	297	BV210419
VDR 1a-36h1 For	GAGTCATCCCTGATCCTTTTTGT				BV210420
VDR 1a-36h1 Rev	TTCTGAGAAACAGATGAAGTGCC	1.5	60	2201	BV210421
VDR 1a-36h2 For	CTGGCAGAGAGGTCAAGAGAC				BV210422
VDR 1a-36h2 Rev	GCTGAGGAGTATCAGAGCTACTG	1.5	60	1,119	BV210423
VDR 1a-36h3 For	GAATGGTGCTTGTCATCGAGG
VDR 1a-36h2 Rev	GCTGAGGAGTATCAGAGCTACTG	1.5	60	651	BV210424
VDR 1a-36h4 Forc	AAGACCAGAGAATTGACAGTTCCTT
VDR 1a-36h2 Rev	GCTGAGGAGTATCAGAGCTACTG	1.5	56	352	BV210425
VDR 1a-36h2 For	CTGGCAGAGAGGTCAAGAGAC
VDR 1a-36h4 Rev	AGGAACTGTCAATTCTCTGGTCTT	1.5	60	768	BV210421
VDR 1a-14h For	GGGGATCCTTCCTATTACTTTCATTACA
VDR 1a-14h Rev	CTTAGACTCACTGTGCAGTGGAGATG	1.5b	60	612	BV210426
VDR 1a-10h For	GGAGGTCATCGACTGCTGGA
VDR 1a-10h Rev	CTAGCTCCGACGAATGGGAAA	1.5	60	535	BV210427
VDR 1a-5h For	ATCTGTGGGATCAGGCTGAGC
VDR 1a-5h Rev	CGCCTTTTGACAAGCAGAGACA	1.5b	60	550	BV210428
VDR 1ad For	TGGTTGATTCCAAGTCAAGATGG
VDR 1ad Rev	CTCCAGCAGTTCTGAGCACCA	1.5b	60	604	BV210429
VDR1d+7h For	CGCTAACACAGTGCTTAGCACTT
VDR1d+7h Rev	AGTAAAATGCCTGCCCAACTG	1.5	60	710	BV210430
1gA-For	TCCTGTCTGGGCTCAGTTG
1gA-Rev	TAGCTTGGTAAGGGTCCAAGTC	1.5b	60	879	BV210431
1gB-For	AATTCACAGTCTATGCTCTGGCT
1gB-Rev	GATCCTCTCAGAACTGGACAATAGT	1.5b	59	896	BV210432
1gC-For	TGAGAGATAGCAGGAAGCAGAAC
1gC-Rev	CTGGCTGCTTACCTGCTTTAC	1.5b	60	967	BV210433
1b-2k For	GACCACACTATCCCACAGAAAGT
1b-2k Rev	GAATGTCCCACCTTGCATAAC	1.5b	59	842	BV210434
1b-gap For	AAGCGGAGTCTGTGCAGAC
1b-gap Rev	AGGTGGCTGCATCTCTTTAGA	1.5b	59	835	BV210435
1c-2kb For	AAGGACCGTCCACTATTGGA
1c-2kb Rev	AAGTCCTTCCCAGCTGACC	1.5b	59	875	BV210436
1c-12h For	CTGAGGAATCAATAAGGCCAG
1c-12h Rev	TGGATCTTAGCAGCTGGCT	1.5b	59	622	BV210413
1c-8h For	TTGTCTATGTCTGCAGGTGGA
1c-8h Rev	CTTTTATCTAAGGCGGAGCG	1.5b	59	816	BV210437
VDR E1c-For	CACTTCTGTTTGCAGTCACTGA
VDR E1c-Rev	GGCTGGATAGGAAACATCAGA	1.5	60	489	BV210438
Exons:
VDR E2-For	ATGCTCTGAGCCAGCTATGTAG
VDR E2-Rev	GAGAGTCAGAGGAACATCTGGA	1.5	60	571	BV210439
VDR E3-For	TGTCTTCTGTTGGAGAAATGGA
VDR E3-Rev	AGTGCATCTGACCCTGGACT	1.5	60	495	BV210440
VDR E4-For	TTTCTTCACACAGTGGAGTGG
VDR E4-Rev	GGCTTTGAGGAAGGTCTACAG	1.5	60	526	BV210441
VDR E5-For	ATCCTGAACAGAACTGGGGTA
VDR E5-Rev	TGATGCTACACAGCTGGAATC	1.5	60	519	BV210442
VDR E6-For	TACTGCCTTATGCTGCTGAAA
VDR E6-Rev	GAGAATCGCTTGAACCTAGGA	1.5	60	557	BV210443
VDR E78-For	CAGCAGGTGTATACCTGTCAAAG
VDR E78-Rev	AGCAGGTCTTTGTCCTTCATACT	1.5	60	634	BV210444
VDR E9-For	CTAGGTCTGGATCCTAAATGCA
VDR E9-Rev	TTAGGTTGGACAGGAGAGAGAA	1.5	60	628	BV210445
3′-UTR:
VDRU1-For	TGAGTGCAGCATGAAGCTAAC
VDRU1-Rev	ATATAACCAGGGCAATGGGAT	1.5	59	580	BV210446
VDRU2-For	TCCTGCCTTACTCACGATAAATAA
VDRU2-Rev	CTAGCTCTTAGCCCTGTGGTG	1.5	60	612	BV210447
VDRU3-For	AAGAATTTTCAGACCCCAGC
VDRU3-Rev	TTTCCACCTGAAGAATTCTGAG	1.5	60	621	BV210448
VDRU4-For	GAGGAATCAGACTTCACACTGC
VDRU4-Rev	AGAGACAGGGTTTCTCCATGTT	1.5	61	592	BV210449
VDRU5-For	GTAGGTGGATCACCTGAGGTC
VDRU5-Rev	AGTAACTGATATTTCAGGAGTTCCC	1.5	61	656	BV210450
VDRU5-For	GTAGGTGGATCACCTGAGGTC
VDRU5-PoA-Rev	CAGATCCAGACTTGGCTCTTT	1.5	60	403	BV210451
VDRU6-For	ATGCTGTTGCCTCATCTATAACA
VDRU6-Rev	ATAATGATTCATCTCCCATAAGGTC	1.5	60	680	BV210452

^aGenBank database accession numbers of the small sequence fragments. The accession numbers of the four long sequence fragments (2.5–9.6 kb; I–IV in fig. 1c) are AY827085–AY827088.

^bAdd 5 × Q-Solution (Qiagen) to the PCR system.

^cSeminested PCR using the fragment generated with primer set VDR 1a-36h2 For and VDR 1a-36h2 Rev as the template.