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Analysis of H19 methylation in patients with BWS and IH. A 1.0-kb fragment of the H19 gene was hybridized to genomic DNA digested with PstI and the methylcytosine-sensitive restriction enzyme SmaI. The upper band represents the 1.8-kb methylated fragment, and the lower band represents the 1.0-kb unmethylated digested fragment. Patient numbers corresponding to those in the tables are shown below the lanes. All DNA specimens were from the normal kidney. fk = fetal kidney.
