Figure 2.

Analysis of LIT1 methylation in patients with BWS and IH. An EST probe from the LIT1 gene was hybridized to genomic DNA digested with BamH1 and the methylcytosine-sensitive restriction enzyme NotI. The upper band represents the 6.0-kb unmethylated fragment, and the lower band represents the 4.2-kb unmethylated digested fragment. Patient numbers corresponding to those in the tables are shown below the lanes. All DNA specimens were from the normal kidney except samples for IH13, IH14, and IH17, which were from the Wilms tumor. fk = fetal kidney.